Journal: Life

Article Title: In Vitro Effects of Cypermethrin and Glyphosate on LPS-Induced Immune Cell Activation

doi: 10.3390/life14010062

Figure Lengend Snippet: Effects of Cypm (reported as C in the graph) on MBT-induced activation in naïve THP-1 and iDCs. ( A , B ) Naïve THP-1 cells (10 6 cells/mL) were treated for 24 h with Cypm (0.4 μg/mL) or with DMSO as vehicle, then stimulated with or without MBT (30 μg/mL) for 24 h. The expression of CD86 ( A ) and the release of IL-8 ( B ), as expressed as pg/mL, are reported. ( C – F ) iDCs (2 × 10 5 cells/mL) were treated for 72 h with Cypm (0.4 μg/mL) plus MBT (30 μg/mL), MBT alone, or with maturation cocktail (rhIL-4, rhGM-CSF, rhTNF-α, and ionomycin) (mDCs), as described in . The expression of CD83 + cells ( C ) and HLA-DR + cells ( D ) as expressed as fold-change (SI) were measured by FACS analysis, and the release of IL-6 ( E ) and IL-18 ( F ), expressed as pg/mL, was measured by ELISA. Each value represents the mean ± SEM, n = 3 independent experiments. Data were analyzed by paired t -test, with * p < 0.05 and ** p < 0.01.

Article Snippet: To obtain immature DCs (iDCs), THP-1 cells were differentiated with 100 ng/mL of rhIL-4 and 100 ng/mL of rhGM-CSF (ImmunoTools, Friesoythe, Germany) for 5 days.

Techniques: Activation Assay, Expressing, Enzyme-linked Immunosorbent Assay